Effect of ageing process on fungiform papillae density

Received: 2017-01-09 | Accepted: 2017-02-24 | Available online: 2017-06-20http://dx.doi.org/10.15414/afz.2017.20.01.31-35Ageing is accompanied by physiological changes in the human oral cavity. These include potential reduction of the fungiform papillae (FP) density which has been associated with impaired taste acuity. Because the studies have demonstrated either contrary or non-significant evidences, we decided to verify the hypothesis about FP reduction and ageing. Total of 150 human living subjects aged 19–87 years participated in the study. The tongues were stained with blue food dye, quantification area with 10 mm diameter was defined, and images of the tongues were captured with digital camera. The number of FP has been scored on each photo by five individual scorers according to a decision tree. The scoring was accomplished in ImageJ open source program using a cell counter plugin. The mean FP density value within all subjects was 18.02 ±15.95 FP per cm-2, the lowest and the highest values were 0.25 ±0.57 FP per cm-2 and 65.00 ±1.66 FP per cm-2 of the tongue tip, respectively. Based on age, the subjects were divided into categories with 6.0 increasing step, in order access effect of ageing on FP densities. Age categories were significantly different in mean FP densities as determined by Welch's F (8, 22.04) = 28.81, P < 0.01). The Games-Howell post hoc test revealed that participants aged 18–24 years had significantly higher FP densities than subjects aged more than 48 years. High degree of intra-groups variance was observed, which could reflect in non-significant differences between the other age groups. Nevertheless, the results were sufficient to support the hypothesis that FP quantity diminish with age.Keywords: fungiform papillae, density, tongue, ageingReferences ADDINSOFT. (2016) XLSTAT: Analyse de données et statistique avec MS Excel. Addinsoft.CORREA, M. et al. (2013). Changes in Fungiform Papillae Density During Development in Humans. Chemical Senses, vol. 38, no. 6, pp. 519-527. doi:http://dx.doi.org/10.1093/chemse/bjt022CVIJANOVIC, N. et al. (2015). Oral and intestinal sweet and fat tasting: impact of receptor polymorphisms and dietary modulation for metabolic disease. Nutrition Reviews, vol. 73, no. 5, pp. 318-334. doi:http://dx.doi.org/10.1093/nutrit/nuu026FISCHER, M. et al. (2013). Factors Related to Fungiform Papillae Density: The Beaver Dam Offspring Study. Chemical Senses, vol. 38, no. 8, pp. 669-677. doi:http://dx.doi.org/10.1093/chemse/bjt033FENG, P., HUANG, L. and WANG, H. 2013. Taste Bud Homeostasis in Health, Disease, and Aging. Chemical Senses, vol. 39, no. 1, pp. 3-16. doi:http://dx.doi.org/10.1093/chemse/bjt059GARNEAU, N. et al. (2014). Crowdsourcing taste research: genetic and phenotypic predictors of bitter taste perception as a model. Frontiers in Integrative Neuroscience, vol. 8, no. 33, pp. 1-8. doi:http://dx.doi.org/10.3389/fnint.2014.00033IKEDA, M. et al. (2007). Causative factors of taste disorders in the elderly, and therapeutic effects of zinc. The Journal of Laryngology and Otology, vol. 122, no. 2, pp. 155-160. doi:http://dx.doi.org/10.1017/s0022215107008833IMOSCOPI, A. et al. (2012). Taste loss in the elderly: epidemiology, causes and consequences. Aging Clinical and Experimental Research, vol. 24, no. 6, pp. 570-579. doi:http://dx.doi.org/10.3275/8520JUST, T. et al. (2006). Contact Endoscopic Comparison of Morphology of Human Fungiform Papillae of Healthy Subjects and Patients with Transected Chorda Tympani Nerve. The Laryngoscope, vol. 116, no. 7, pp. 1216-1222. doi:http://dx.doi.org/10.1097/01.mlg.0000224509.61099.29KONSTANTINIDIS, I. et al. (2010). Effects of smoking on taste: Assessment with contact endoscopy and taste strips. The Laryngoscope, vol. 120, no. 10, pp. 1958-1963. doi:http://dx.doi.org/10.1002/lary.21098NUESSLE, T. et al. (2015). Denver Papillae Protocol for Objective Analysis of Fungiform Papillae. Journal of Visualized Experiments, vol. 100. doi:http://dx.doi.org/10.3791/52860PAVLIDIS, P. et al. (2012). Age-related Changes in Electrogustometry Thresholds, Tongue Tip Vascularization, Density, and Form of the Fungiform Papillae in Humans. Chemical Senses, vol. 38, no. 1, pp. 35-43. doi:http://dx.doi.org/10.1093/chemse/bjs076CHANDRASHEKAR, J. et al. (2006). The receptors and cells for mammalian taste. Nature, vol. 444, no. 7117, pp. 288-294. doi:http://dx.doi.org/10.1038/nature05401SEGOVIA, C. et al. (2002). A quantitative study of fungiform papillae and taste pore density in adults and children. Developmental Brain Research, vol. 138, no. 2, pp. 135-146. doi:http://dx.doi.org/10.1016/s0165-3806(02)00463-7SHAHBAKE, M. et al. (2005). Rapid quantitative assessment of fungiform papillae density in the human tongue. Brain Research, vol. 1052, no. 2, pp. 196-201. doi:http://dx.doi.org/10.1016/j.brainres.2005.06.031ZHANG, G. et al. (2008). The Relationship between Fungiform Papillae Density and Detection Threshold for Sucrose in the Young Males. Chemical Senses, vol. 34, no. 1, pp. 93-99. doi:http://dx.doi.org/10.1093/chemse/bjn059ZUNIGA, J. et al. (1993). Taste performance on the anterior human tongue varles with fungiform taste bud density. Chem Senses, vol. 18, no. 5, pp. 449-460. doi:http://dx.doi.org/10.1093/chemse/18.5.44

The prevalence of Salmonella infections in laying hen flocks producing eggs and their impact on the public health

Since 2008, Slovakia has implemented the National control program of Salmonella infections in laying hen flocks. This program requires the farm operators to monitor and investigate the invasive types of Salmonella (S. Enteritidis and S. Typhimurium) according to STN ISO 6579. The aim of this study was to perform a microbiological examination of dust and chicken droppings samples of laying hens in the Trenčín region for the presence of Salmonella by Horizontal method according to STN ISO 6579: 2002, to compare results with the statistics across Slovakia and selected EU countries and to evaluate the impact of official controls of salmonellosis in animals and humans.  In the years 2009 - 2013 in the Trenčín region, 730 samples of dust from the conveyor belts and droppings of laying hens were taken to determine the prevalence of Salmonella in individual rearings. In these years, the incidence of positive samples was found from 0% to 29.17%. For the period from 2009 till 2013 was reported 22833 salmonellosis cases in human population of Slovakia, while in the Trenčín region it was 2636. Five-year EU-trend (2009 - 2013) showed a statistically significant decrease of salmonellosis occurence (with a mean reduction of 12% per year). The Wilcoxon signed-rank test was performed in order to provide in-depth epidemiological assessment of salmonellosis cases in Trenčin region in relation to selected characters: etiological agens, transmission mechanism, age, location as well as seasonality of infection

Comparison of phytoestrogens dietary intake from vegetables and fruit in selected population in Slovakia

Phytoestrogens are compounds that are naturally present in almost all plant foods to a varying degree. They include several different classes of chemical compounds known as isoflavones, coumestans and lignans. In our work we analyzed intake of phytoestrogens is based upon our answer questionaries' in different ages. Evaluating health effects of phytoestrogens is difficult and depends on numerous factors, including the kind and dose (amount) of phytoestrogens eaten and the age, gender, and health of the person. We are exposed daily to highly variable amounts of phytoestrogens. While adults are eating a vegetarian diet or those taking dietary supplements containing phytoestrogens have high levels of exposure, infants drinking soy-based formula have the highest exposure levels by far. Accurate information about dietary phytoestrogens is therefore important but there is very limited data concerning food contents. In this study, we analyzed the phytoestrogen content in fresh and processed fruits and vegetables. The comprehensive database of phytoestrogen content determined simultaneously in vegetables and fruits foods has been developed. The important source of phytoestrogens in Slovak men and women is garlic. Phytoestrogen intake of fruits in men as an in woman is very low. Slovak populations consume a lot of fruits but the total intake is low due to the lower content of phytoestrogens

Influence of different curing methods on the fatty acid composition in sausages prepared from red deer meat

These curing agents play a decisive role in obtaining the specific sensory properties, stability and hygienic safety of products such as fermented sausages, ham and, more recently, emulsion type of sausages. The effect of using two different curing agents (sodium chloride and nitrate) on fatty acid compounds in dry-cured deer meat was investigated in our study. The concentration of free fatty acids in the fat depends on the hydrolytic activity of the lipases, the microbial metabolic processes, and the oxidative reactions that work on the free fatty acids released in the lipolysis. The main identified fatty acids in all different types of curing were palmitic acid (16 : 0), oleic acid (c18 : 1 cis-9), stearic acid (C18 : 0). The resulting n-6/n-3 PUFA ratio in the muscle samples of red deer showed no variation in different types of curing and was beneficially low within the range of 3.9 : 1 and 4.49 : 1. Total free fatty acids, whether saturated, monounsaturated or polyunsaturated fatty acids, did not increased (p &gt;0.05) greatly through the processing of dry-cured deer meat. Also there was no effect of curing method on fatty acids composition in two different muscles Semitendinosus muscle (ANOVA, p &gt;0.05, F - 0.003, F crit. - 3.041) and Triceps brachii muscle (ANOVA, p &gt;0.05, F - 0.05, F crit. - 3.01). There were found no significant (p &gt;0.05) differences between fatty acids content in sausages prepared by brining in NaCl and Nitrate salt. The present study revealed that game meat can function as a good source of bioactive compounds that are essential for human nutrition

Identification of differences in chemical composition among whole stick and sliced Nitran salamis trough principal component analysis

The subject of this work was to examine differences in chemical composition of sliced and whole stick Nitran salamis, purchased from various manufacturers. Nitran salamis are traditional dry fermented meat products of Slovak origin. Taking into account variations in raw materials, production process and potential adulteration, differences in chemical composition within one brand of salami from different manufacturers might be expected. Ten salamis were determined for basic chemical composition attributes and Principal Component Analysis was applied on data matrix to identify anomalous ones. It has been shown that six attributes, namely: protein without collagen of total protein, total protein, total meat, total fat, collagen of total protein and NaCl, were the most important for salamis as first two Principal Components together explained 70.16% of variance among them. Nitran D was found to be the most anomalous salami, as had the lowest value of protein without collagen of total protein (14.14% ±0.26%), total protein (17.42% ±0.44%), total meat (120.29% ±0.98%) and the highest one of total fat (50.85% ±0.95%), collagen of total protein (18.83% ±0.50%) and NaCl (9.55% ±1.93%), when compared to its whole stick variant Nitran C and other samples. In addition to collagen of total protein content, Nitran D together with Nitran A, F and H did not satisfied the legislatively determined criterion, which is ≤16%. This suggested that extra connective tissues were added to intermediate products, which resulted in high variability and inferior quality of final products. It is a common practice in the meat industry to increase the protein content or water binding properties of meat products

Texture quality of muskmelons (Cucumis melo L.) From different retailers during storage

The subject of study was to assess and to compare the effect of storage time on flesh firmness and skin strength in muskmelons (Cucumis melo L.) obtained from supermarket and marketplace. Texture quality of fruit is considered to be the major determinant for customer preference that depends on harvesting maturity and proper storage conditions of fruit and its fresh-cuts. Changes in flesh firmness and skin strength were measured periodically in stored fresh-cut fruits in both groups for initial, 2nd, 5th and 6th day. Analysis of basic physical parameters revealed that muskmelons from marketplace had higher height and width perimeter and therefore also a higher weight, compared to those ones from supermarkets. Textural analysis pointed out to significant differences (p &lt;0.05) in flesh firmness among initial day group and all tested groups from 2nd, 5th and 6th day in muskmelons from supermarket. In marketplace muskmelon group was observed significant difference (p &lt;0.05) between samples from initial day and 5th day. Within the skin strength, there were demonstrated significant differences (p &lt;0.05) between initial day and most of remaining storage days in both supermarket and marketplace muskmelons groups. The data for flesh firmness and skin strength were used in linear regression analysis, in order to evaluate trends during storage period. The correlation coefficients of linear model describing relationship between storage time and skin strength for the group of supermarket and marketplace muskmelons were r = -0.828 and r = -0.780, respectively, which indicated approximately equal relationship between time and skin strength in both tested groups. A strong inverse correlation (r = -0.816) between time and flesh firmness in the group of supermarket muskmelons was noticed. In the group of marketplace muskmelons, there was observed weaker inverse correlation (r = -0.441) within this relation, compared to commercial ones. The model revealed that the muskmelons from marketplace retailers tend to maintain the flesh firmness for a longer time than did commercial ones. The melon flesh quality was markedly changing during storage period and highly depends on the muskmelon origin

Authentication of caprine milk and cheese by commercial qPCR assay

The objective of the study was to investigate potential adulteration of commercial caprine milks and cheeses with bovine milk using commercial qPCR assay. The assay comprised of bovine-, ovine- and caprine-specific primers and TaqMan probe and mammalian internal control. Specificity, sensitivity, linearity, reproducibility and efficiency of the bovine assay were tested as well. Specificity was verified by running reaction on the DNA of other milk-producing species (caprine and ovine) and made-up bovine-caprine (v/v) milk mixes. In both experiments, a bovine DNA fragment was amplified whereas no amplification was obtained from the other species. Sensitivity, linearity, reproducibility and efficiency were tested on 10-fold dilution series of 10 ng bovine DNA. The assay has shown good linearity (R2 = 0.983) within whole range, with efficiency of 86% and excellent reproducibility (SD around the CT for the technical replicates &lt;0.5). The sensitivity was adequate, as calculated LOD and LOQ were 1.44 pg and 2.94 pg of bovine DNA, respectively. Finally, the assay was used to authenticate 5 caprine milk samples and 5 caprine cheese samples, purchased from local supermarkets. Totally, 1 milk sample has shown the fluorescence signal, which exceeded baseline in cycle 39.01 &plusmn;0.69. However, the signal was above LOD and LOQ suggesting that there could not be unambiguously declared any adulteration with bovine milk. Amplification of bovine-specific DNA was not observed in the other samples indicating products were not adulterated. The commercial qPCR assay has proved that real-time PCR assays, as well as DNA-based techniques in a general, are the excellent and reliable tools for fighting with frauds in the food industry and protecting the public health.&nbsp

Detection of ovine milk adulteration using taqman real-time pcr assay

Food safety, quality and composition have become the subjects of increasing public concern. To prevent fraud and enhance quality assurance, credible analysis of dairy products is crucial. Bovine milk is more widely available and cheaper than milk of sheep and goat. Bovine milk is also processed in large quantities to produce a range of dairy produce. DNA-based methods have proven to be more reliable, because of the stability of DNA under the conditions of high temperature, high pressure, and chemical treatment used during the processing of some food products. The commercial InnuDETECT cheese assay based on the principle TaqMan real-time PCR systems have been tested for the identification and quantification of bovine DNA in ovine milk samples. DNA was extracted using the InnuPREP DNA Mini Kit and quantified by the QuantiFluor dsDNA system. The assay showed good linearity, with correlation coefficient of R2 = 0.983 and efficiency of 86%. The internal control amplified fragment from different mammalian species (cow, sheep and goat), with similar CT values. Detection of bovine DNA in milk mixtures was achieved even in samples containing 0.5% of bovine milk. The InnuDETECT cheese assay has been successfully used to measure bovine DNA in ovine milk, and will prove useful for bovine species identification and quantitative authentication of animal-derived products

Protein quality chicken meat after feeding with active substances of citrus fruits and diclazuril and salinomycin sodium

The purpose of this study was an experimental investigation of the influence of active substances obtained mainly from citrus fruits in the experimental feed mixtures, and diclazuril and salinomycin sodium in the control feed mixtures of broiler chickens on productive efficiency and protein quality of the breast and thigh muscles. In vivo experiment was carried out with hybrid chickens Cobb 500. Basic feed mixtures were equal a soy cereal type for experimental and control group. Indicators of productive efficiency were measured and calculated, and protein, lysine and methionine contents in the breast and thigh muscles were measured by the method of FT IR, Nicolet 6700. Active substances obtained mainly from citrus fruits confirmed a statistically significant (p ˂0.05) positive effect on the body weight gain; tended to slightly increase feed intake per bird, protein, energy, lysine and methionine intake per bird; slightly decrease feed intake per 1 kg of body weight gain, protein, energy, lysine and methionine intake per 1 kg of body weight gain; slightly increase protein efficiency ratio and energy efficiency ratio. Additive substances used in the feed mixtures did not have a statistically significant effect on protein, lysine and methionine contents in the breast and thigh muscles but displayed a strong positive, statistically significant relation between lysine and methionine in them

Thermo-degradative changes of rapeseed and sunflower oils during deep-frying French fries

The purpose of this study was to investigate changes in TPCs, acid value and peroxide value as well as fatty acids composition in edible oils during french fries production. Lower TPCs content was found in rapeseed oil (3.3%) and the threshold (24%) was achieved on the fourth day. The total time for the deterioration of deep-frying rapeseed oil was 23½ hours. On the contrary, in fresh sunflower oil at the first day was TPCs content 5.5% and the limit of 24% was reached on the third day. The total time for the deterioration of deep-frying sunflower oil was 17½ hours. The results indicated significant differences (<0.05) in TPCs content between rapeseed and sunflower oils during deep-frying process. At the beginning of deep-frying French fries in rapeseed oil, the acid number was 0.374 mg KOH.g-1 and 1.271 mg KOH.g-1 at the fourth day of deep-frying. The measured peroxide value was 4.3 mEq O2.kg-1 at the beginning and at the end of deep-frying 10.5 mEq O2.kg-1. The initial peroxide and acid values were higher in sunflower oil compared with rapeseed oil, respectively. It should be note, then the acid values and peroxide values, respectively, in the two fresh oils used in this study were below the limit of refined oil according to Slovak legislation (peroxide value - not more than 10 mEq O2.kg-1, acid value - not more than 0.6 mg KOH.g-1). However, detected values varied during deep-frying process. Monounsaturated fatty acids were predominantly observed in fresh rapeseed oil (61.22%) wherever in sunflower oil they were much lower (29.77%). A slight increase of MUFA was found in both oils. The initial content of saturated fatty acids in rapeseed oil was 6.94%, in fresh sunflower oil was observed slightly higher content of SFA (10.37%). The major groups of fatty acids in fresh sunflower oil were polyunsaturated fatty acids (PUFA) which have in principle a significant effect on oil deterioration. A slight decrease of PUFA was observed in both oils throughout the frying period. The content of PUFA was reduced by about 9.42% in rapeseed oil and by 10.8% in sunflower oil. The initial content was 28.14% and 58.91%, respectively